DETERMINATION OF ANTIOXIDANT ACTIVITY AND PHENOLIC COMPOUND CONTENT OF BLACK CARROT CALLUS CULTURE

ILHAMI KARATA޹ , Fatih POLAT² , RAHIME KARATA޳ , TARIK DAL⁴ , MAHFUZ ELMASTAŞ⁵

Callus culture, a method of plant tissue culture, is an alternative technique for the production of phenolic compounds that many industries need. In this study, antioxidant capacity, total phenolic, flavonoid and anthocyanin content of callus obtained from black carrot (Daucus carota ssp. Sativus var. Atrorubens alef.) were investigated. The antioxidant capacities of the extracts were determined by the free radical scavenging activity (DPPH) and the ferric reducing antioxidant power (FRAP) methods. The callus was derived from the MS medium (Murashige ve Skoog containing 2 mg/L 2.4-D, 0.2mg/L BAP, 30g/L sucrose and 2g/L phytagel using hypocotyl segments of plants grown in sterile conditions. The black carrot calli exhibited high DPPH free radical scavenging activity and FRAP activity in addition to high phenolic compound contents.

Keywords
Antioxidant Activity, Anthocyanin, Black Carrot, Callus Culture, Flavonoid, Phenolic Compound,