THE CYTOTOXIC, APOPTOTIC AND NECROTIC EFFECCTS OF LAETIPORUS SULPHUREUS (BULL.) MURRILL

Aydın KESKİN¹ , Perihan GÜLER² , Mustafa TÜRK³

In the study, cytotoxicity, apoptotic, necrotic effects of laetiporus sulphureus were investigated. Taken parts from the basidiocarps in the laboratory were developed with tissue culture on malt extract agar. The primary and the secondary mycelium were obtained by incubation at 27°C in the dark for 10 d. Three pellets were taken from the secondary mycelium and were completed their growth in 7 d at 140 rpm at 27°C in nutrient broth. The samples were prepared for cytotoxic, apoptotic, necrotic, studies by extracting at 80°C and 1 h in ethyl alcohol at soxlet device. In cytotoxic studies, sample concentrations for extract MCF-7 and L929 fibroblast cells were applied as 0.5mg/ml, 0.25mg/ml, 0.125mg/ml, 0.0625mg/ml, 0.03125mg/ml respectively. The WST-1 solution was added and the absorbance was measured in a microplate reader at a wavelength of 440 nm at the end of the incubation. Apoptosis and necrosis were detected in same cell line as the double staining experiment. Binary staining solution was prepared and MCF-7 and L929 fibroblast fibroblast cells were applied at the same concentrations. As a result of incubation, the measurement was done at fluorescense microscopy at 20X magnification in FITC and DAPI filters. Ten percent toxicity was observed at 0.25mg/ml dosage used in MCF-7 cell line, At all dosages used, viability rates were increased in L929 Fibroblast cell lines. Apoptotic and necrotic effects were observed in the samples.

Keywords
Laetiporus sulphureus, Cancer, Cytotoxicity, Apoptotic Effects, Necrotic Effects,