• icon+90(533) 652 66 86
  • iconnwsa.akademi@hotmail.com
  • icon Fırat Akademi Samsun-Türkiye

Article Details

Article Details

  • Article Code : FIRAT-AKADEMI-8354-5462
  • Article Type : Araştırma Makalesi
  • Publication Number : 5A0166
  • Page Number : 59-67
  • Doi : 10.12739/NWSA.2022.17.2.5A0166
  • Abstract Reading : 1099
  • Download : 188
  • Share :

  • PDF Download

Issue Details

  • Year : 2022
  • Volume : 17
  • Issue : 2
  • Number of Articles Published : 3
  • Published Date : 1.04.2022

Cover Download Context Page Download
Dergi Kapağı
Ecological Life Sciences

Serial Number : 5A
ISSN No. : 1308-7258
Release Interval (in a Year) : 4 Issues

Işıl Canan ÇİÇEK ÇİMEN1 , Osman SERDAR2

Keywords

EFFECT OF METALLOTHIONEIN LEVELS IN Gammarus pulex EXPOSED TO COPPER AND COPPEROXIDE NANOPARTICLES

Işıl Canan ÇİÇEK ÇİMEN1 , Osman SERDAR2

With the increase in the use of products containing nanoparticles (NP) (<100nm), the food chain including aquatic systems and organisms is at risk due to the effect of NPs released as a result of anthropogenic activities. In this study, metallothionine (MT) biomarker responses were determined by exposing Gammarus pulex, an indicator species living in freshwaters, to metal-based copper (Cu) and copper oxide (CuO) NPs. In the experiment, 4 groups (Control, 10ppm, 20ppm, 40ppm) were formed and studied with three replications. It was determined that the Cu-NP application samples caused an increase (p<0.05) in MT levels compared to the control at the 24th and 96th hours samples. It was determined that CuO applied samples caused increases in MT levels compared to the control group (p<0.05), but this increase in 96-hour samples was lower than the increase at the 24th hour samples. As a result of the study, it is predicted that Cu and CuO NPs cause an increase in MT levels in the organism, and this increase may cause oxidative stress.

Keywords
Metallothiyonein, Gammarus pulex, Biomarker, Copper Nanoparticle,, Copper Oxide Nanoparticle,

Details
   

Authors

Işıl Canan ÇİÇEK ÇİMEN (1)

Munzur Üniversitesi
isilcanancicek@gmail.com | 0000-0002-7427-8211

Osman SERDAR (2) (Corresponding Author)
Munzur Üniversitesi
oserdar@munzur.edu.tr | 0000-0003-1744-8883

Supporting Institution

 :

Project Number

 :

Thanks

 :
References
[1] Swiergosz-Kowalewska, R., (2001). Cadmium distribution and toxicity in tissues of small rodents. Microscopy research and technique, 55(3):208-222.

[2] Dameron, C.T. and Harrison, M.D., (1998). Mechanisms for protection against copper toxicity. The American Journal of Clinical Nutrition, 67(5):1091-1097.

[3] Brüwer, M., Schmid, K.W., Metz, K.A., Krieglstein, C.F., Senninger, N., and Schürmann, G., (2001). Increased expression of metallothionein in inflammatory bowel disease. Inflammation Research, 50(6):289-293.

[4] Webb, M., (1987). Toxicological significance of metallothionein. Metallothionein II, 109-134.

[5] Tomita, Y., Kondo, Y., Hara, M., Yoshimura, T., Igarashi, H., and Tagami, H., (1992). Menkes’ disease: report of a case and determination of eumelanin and pheomelanin in hypopigmented hair. Dermatology, 185(1):66-68.

[6] Sanz-Nebot, V., Andón, B., and Barbosa, J., (2003). Characterization of metallothionein isoforms from rabbit liver by liquid chromatography coupled to electrospray mass spectrometry. Journal of Chromatography B, 796(2):379-393.

[7] Muralisankar, T., Bhavan, P.S., Radhakrishnan, S., Seenivasan, C., and Srinivasan, V., (2016). The effect of copper nanoparticles supplementation on freshwater prawn Macrobrachium rosenbergii postlarvae. Journal of Trace Elements in Medicine and Biology, 34:9–49.

[8] Turnlund, J., (1994). Copper, in: M.E. Shils, J.A. Olson, M. Shike (Eds.), Modern Nutrition in Health and Disease, Volume:1, (8th Edition), Lea & Febiger Malvern, USA, pp:231–241.

[9] Watanabe, T., Kiron, V., and Satoh, S., (1997). Trace minerals in fish nutrition, Aquaculture, 151:185–207.

[10] Lall, S.P., (2002). The minerals (Editors: J.E. Halver, R.W. Hardy), Fish Nutrition, Academic Press, New York, pp:259–308.

[11] Lorentzen, M., Maage, A., and Julshamn, K., (1998). Supplementing copper to a fish meal-based diet fed to Atlantic salmon parr affects liver copper and selenium concentrations. Aquaculture Nutrition, 4:67–72.

[12] Lee, M. and Shiau, S., (2002). Dietary copper requirement of juvenile grass shrimp Penaeus monodon and effects on non-specific immune response. Fish & Shellfish Immunology, 13(4):259–270.

[13] Wang, W., Mai, K., Zhang, W., Ai, Q., Yao, Q., Li, H., and Liufu, Z., (2009). Effects of dietary copper on survival growth and immune response of juvenile abalone Haliotisdiscus hannaiIno, Aquaculture, 297:122–127.

[14] Shao X.P., Liu, W.B., Lu, K.L., Xu, W.N., Zhang, W.W., Wang, Y., and Zhu, J., (2012). Effects oftribasic copper chloride on growth copper status antioxidant activities immune responses and intestinal micro flora of blunt snout bream (Megalobrama amblycephala) fed practical diets. Aquaculture, 338–341:154–159.

[15] Sun, S., Qin, J., Yu, N., Ge, X., Jiang, H., and Chen, L., (2013). Effect of dietary copper on thegrowth performance, non-specific immunity and resistance to Aeromonas hydrophila of juvenile Chinese mitten crab, Eriocheir sinensis. Fish Shellfish Immunology, 34:1195–1201.

[16] Clearwater, S.J., Farag, A.M., and Meyer, J.S., (2002). Bioavailability and toxicity of diet borne copper and zinc to fish. Comparative Biochemistry and Physiology, 132C:269–313.

[17] Gomez, J., Martínez, A.C., Gonzalez, A., and Rebollo, A., (1998). Dual role of Ras and rho proteins: At the cutting edge of life and death. Immunology and Cell Biology, 76:125-134.

[18] Fahmy, B. and Cormier, S.A., (2009). Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells. Toxicology in Vitro, 23:1365-1371.

[19] Wang, Z., Li, J., Zhao, J., and Xing, B., (2011). Toxicity and internalization of CuO nanoparticles to prokaryotic alga Microcystis aeruginosa as affected by dissolved organic matter. Environmental Science & Technology, 45(14):6032-6040.

[20] Melegari, S.P., Perreault, F., Costa, R.H.R., Popovic, R., and Matias, W.G., (2013). Evaluation of toxicity and oxidative stress induced by copper oxide nanoparticles in the green alga Chlamydomonas reinhardtii. Aquatic Toxicology, 142–143:431–440.

[21] Rotini A., Gallo, A., Parlapiano, I., Berducci, M.T., Boni, R., Tosti, E., Prato, E., Maggi, C., Cicero, A.M., Migliore, L., and Manfra, L., (2018). Insights into the CuO nanoparticle ecotoxicity with suitable marine model species. Ecotoxicology and Environmental Safety, 147:852–860.

[22] Cimen, I.C.C., Danabas, D., and Ates, M., (2020). Comparative effects of Cu (60–80nm) and CuO (40nm) nanoparticles in Artemia salina: Accumulation, elimination and oxidative stress. Science of the Total Environment, 717:137230.

[23] Park, J., Kim, S., Yoo, J., Lee, J.S., Park, J.W., and Jung, J., (2014). Effect of salinity on acute copper and zinc toxicity to Tigriopus japonicus: the difference between metal ions and nanoparticles. Marine Pollution Bulletin, 85(2):526–531.

[24] Rossetto, A.L., Melegari, S.P., Ouriques, L.C., and Matias, W.G., (2014). Comparative evaluation of acute and chronic toxicities of CuO nanoparticles and bulk using Daphnia magna and Vibrio fishery. Science of the Total Environment, 490:807–814.

[25] Wu, B., Torres-Duarte, C., Cole, B.J., and Cherr, G.N., (2015). Copper oxide and zinc oxidenanomaterials act as inhibitors of multidrug resistance transport in sea urchin embryos: their role as chemosensitizers. Environmental Science Technology, 49(9):5760–5770.

[26] Huber, D.L., (2005). Synthesis, properties, and applications of iron nanoparticles. Small 1:482-501.

[27] Colvin, V.L., (2003). The potential environmental impact of engineered nanomaterials. Nature Biotechnology, 21:1166-1170.

[28] Galdiero, S., Falanga, A., Vitiello, M., Cantisani, M., Marra, V., and Galdiero, M., (2011). Silver nanoparticles as potential antiviral agents. Molecules, 16:8894–8918.

[29] Rand, G.M., (Ed.)., (1995). Fundamentals of aquatic toxicology: effects, environmental fate and risk assessment. CRC press.

[30] Serdar, O., (2019). The effect of dimethoate pesticide on some biochemical biomarkers in Gammarus pulex. Environmental Science and Pollution Research, 26(21):21905-21914.

[31] URL-1, (2022). https://www.caymanchem.com/product/10008659 (Date of access: 12.10.2021).

[32] Geffard, A., Geffard, O., Amiard, J.C., His, E., and Amiard-Triquet, C., (2007). Bioaccumulation of metals in sediment elutriates and their effects on growth, condition index, and metallothionein contents in oyster larvae. Archives of Environmental Contamination and Toxicology, 53(1):57-65.

[33] Thomas, J.P., Bachowski, G.J., and Girotti, A.W., (1986). Inhibition of cell membrane lipid peroxidation by cadmium-and zinc-metallothioneins. Biochimica et Biophysica Acta (BBA)-General Subjects, 884(3):448-461.

[34] Vašák, M., (2005). Advances in metallothionein structure and functions. Journal of Trace Elements in Medicine and Biology, 19(1):13-17.

[35] Amiard, J.C., Amiard-Triquet, C., Barka, S., Pellerin, J., and Rainbow, P.S., (2006). Metallothioneins in aquatic invertebrates: Their role in metal detoxification and their use as biomarkers. Aquatic toxicology, 76(2):160-202.

[36] Torres, P., Tort, L., and Flos, R., (1987). Acute toxicity of copper to mediterranean dogfish. Comparative Biochemistry and physiology. C, Comparative Pharmacology and Toxicology, 86(1):169-171.

[37] Halliwell, B. and Gutteridge, J.M., (2015). Free radicals in biology and medicine. Oxford university press, USA.

[38] Viarengo, A., (1989). Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level. Aquatic Science, 1(2):295-317.

[39] Ringwood, A.H., Conners, D.E., and DiNovo, A., (1998). The effects of copper exposures on cellular responses in oysters. Marine Environmental Research, 46(1-5):591-595.

[40] Langston, W.J., and Bebianno, M.J. (Eds.). (1998). Metal metabolism in aquatic environments (No:7). Springer Science & Business Media.

[41] Livingstone, D.R., (2001). Contaminant-stimulated reactive oxygen species production and oxidative damage in aquatic organisms. Marine Pollution Bulletin, 42(8):656-666.

[42] Correia, A.D., Lima, G., Costa, M.H., and Livingstone, D.R., (2002). Studies on biomarkers of copper exposure and toxicity in the marine amphipod Gammarus locusta (Crustacea): I. Induction of metallothionein and lipid peroxidation. Biomarkers, 7(5):422-437.

[43] Zhao, S., Meng, F., Fu, H., Xiao, J., and Gao, Y. (2010). Metallothionein levels in gills and visceral mass of Ruditapes philippinarum exposed to sublethal doses of cadmium and copper. In 2010 International Conference on Challenges in Environmental Science and Computer Engineering, 2:181-186, IEEE.

[44] Lundebye, A.K. and Depledge, M.H., (1998). Automated interpulse duration assessment (AIDA) in the shore crab Carcinus maenas in response to copper exposure. Marine Biology, 130(4):613-620.

[45] Pedersen, S.N., Pedersen, K.L., Hojrup, P., Knudsen, J., and Depledge, M.H., (1998). Induction and identification of cadmium, zinc-and copper-metallothioneins in the shore crab Carcinus maenas (L.). Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 120(2):251-259.

[46] Schlenk, D. and Brouwer, M., (1991). Isolation of three copper metallothionein isoforms from the blue crab (Callinectes sapidus). Aquatic toxicology, 20(1-2):25-33.

[47] Pedersen, S.N., Lundebye, A.K., and Depledge, M.H., (1997). Field application of metallothionein and stress protein biomarkers in the shore crab (Carcinus maenas) exposed to trace metals. Aquatic Toxicology, 37(2-3):183-200.

[48] Engel, D.W., Brouwer, M., and Mercaldo-Allen, R., (2001). Effects of molting and environmental factors on trace metal body-burdens and hemocyanin concentrations in the American lobster, Homarus americanus. Marine Environmental Research, 52(3):257-269.

[49] Brouwer, M., Winge, D.R., and Gray, W.R., (1989). Structural and functional diversity of copper-metallothioneins from the American lobster Homarus americanus. Journal of Inorganic Biochemistry, 35(4):289-303.

[50] Canli, M., Stagg, R.M., and Rodger, G., (1997). The induction of metallothionein in tissues of the Norway lobster Nephrops norvegicus following exposure to cadmium, copper and zinc: The relationships between metallothionein and the metals. Environmental Pollution, 96(3):343-350.

[51] Barka, S., Pavillon, J.F., and Amiard, J.C., (2001). Influence of different essential and non-essential metals on MTLP levels in the copepod Tigriopus brevicornis. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 128(4):479-493.

[52] Vellinger, C., Gismondi, E., Felten, V., Rousselle, P., Mehennaoui, K., Parant, M., and Usseglio-Polatera, P., (2013). Single and combined effects of cadmium and arsenate in Gammarus pulex (Crustacea, Amphipoda): understanding the links between physiological and behavioural responses. Aquatic Toxicology, 140:106-116.

[53] Pedersen, S.N. and Lundebye, A.K., (1996). Metallothionein and stress protein levels in shore crabs (Carcinus maenas) along a trace metal gradient in the Fal Estuary (UK). Marine Environmental Research, 42(1-4):241-246.

[54] Berthet, B., Mouneyrac, C., Pérez, T., and Amiard-Triquet, C., (2005). Metallothionein concentration in sponges (Spongia officinalis) as a biomarker of metal contamination. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 141(3):306-313.

[55] Shariati, F. and Shariati, S., (2011). Review on methods for determination of metallothioneins in aquatic organisms. Biological Trace Element Research, 141(1):340-366.

[56] Geffard, A., Amiard, J.C., and Amiard-Triquet, C., (2002a). Use of metallothionein in gills from oysters (Crassostrea gigas) as a biomarker: seasonal and intersite fluctuations. Biomarkers, 7(2):123-137.

[57] Geffard, A., Geffard, O., His, E., and Amiard, J.C., (2002b). Relationships between metal bioaccumulation and metallothionein levels in larvae of Mytilus galloprovincialis exposed to contaminated estuarine sediment elutriate. Marine Ecology Progress Series, 233:131-142.

[58] Geffard, A., Amiard-Triquet, C., and Amiard, J.C., (2005). Do seasonal changes affect metallothionein induction by metals in mussels, Mytilus edulis?. Ecotoxicology and Environmental Safety, 61(2):209-220.

[59] Bayhan, T., (2015). Büyük Menderes deltasından avlanan kefal (Leuciscus cephalus) ve levreklerde (Perca fluviatilis) Cu, Zn ve Cd düzeylerinin belirlenmesi ve metallotiyonin ile ilişkisinin araştırılması (Master's thesis). Aydın: Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü, Türkiye.

[60] Kagi, J.H.R., Himmelhoch, S.R., Whanger, P.D., Bethune, J.L., and Vallee, B.L., (1974). Equine hepatic and renal metallothioneins: Purification, molecular weight, amino acid composition, and metal content. Journal of Biological Chemistry, 249(11):3537-3542.

[61] Vallee, B.L., (1995). The function of metallothionein. Neurochemistry International, 27(1):23-33.