• icon+90(533) 652 66 86
  • iconnwsa.akademi@hotmail.com
  • icon Fırat Akademi Samsun-Türkiye

Article Details

  • Article Code : FIRAT-AKADEMI-9322-5717
  • Article Type : Araştırma Makalesi
  • Publication Number : 5A0225
  • Page Number : 1-9
  • Doi : 10.12739/NWSA.2025.20.1.5A0225
  • Abstract Reading : 103
  • Download : 38
  • Share :

  • PDF Download

Issue Details

  • Year : 2025
  • Volume : 20
  • Issue : 1
  • Number of Articles Published : 1
  • Published Date : 1.01.2025

Cover Download Context Page Download
Ecological Life Sciences

Serial Number : 5A
ISSN No. : 1308-7258
Release Interval (in a Year) : 4 Issues

TATLI SU İSTAKOZU (Pontastacus leptodactylus Esch. 1823)’NUN OKSİDATİF STRESİ ÜZERİNE VİTAMİN A ve C’NİN ETKİSİ

ÖZDEN BARIM ÖZ1 , Bünyamin ÖZDEMİR2

Bu araştırmada tatlı su istakozu (Pontastacus leptodactylus)’nun metabolik ihtiyacını karşılayan kontrol rasyonuna ilave edilen vitamin C (VC) ve A (VA)’nin hepatopancreas, gonad, kas ve solungaç dokusunda oksidatif stres ve antioksidan savunma (süperoksit dismütaz (SOD), glutatyon peroksidaz (GSH-Px), redükte glutatyon (GSH)) üzerine etkileri belirlendi. Bu amaçla hazırlanan kontrol rasyonuna VC (200 mg kg-1) ve VA (200 mg kg-1) ilave edilerek araştırma grupları oluşturuldu. P. leptodactylus su sıcaklığına göre ayarlanan oranlarda bu rasyonlarla 30 gün süresince beslendi. Araştırma sonucunda P. leptodactylus’un rasyonuna VC ve VA ilave edilmesinin hepatopankreas (p<0,001) dokusundaki malondialdehit seviyesini istatistiksel açıdan önemli derecede azalttığı belirlendi. Ayrıca SOD, GSH-Px aktivitelerinin ve GSH düzeylerinin doku özelliğine göre değiştiği tespit edildi.

Keywords
P. leptodactylus, MDA, SOD, GSH-Px, GSH,

EFFECT OF VITAMIN A AND C ON OXIDATIVE STRESS IN FRESHWATER LOBSTER (Pontastacus leptodactylus Esch. 1823)

ÖZDEN BARIM ÖZ1 , Bünyamin ÖZDEMİR2

In this study, the effects of vitamins C (VC) and A (VA) added to the control diet meeting the metabolic needs of freshwater crayfish (Pontastacus leptodactylus) on oxidative stress and antioxidant defense (superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), reduced glutathione (GSH)) in gonad, hepatopancreas, gill and muscle tissues were determined. For this purpose, VC (200 mg kg-1) and VA (200 mg kg-1) were added to the prepared control diet to form research groups. P. leptodactylus was fed with these diets at rates adjusted according to water temperature for 30 days. As a result of the research, it was determined that the addition of VC and VA to the diet of P. leptodactylus prevented the malondialdehyde level in hepatopancreas (p<0.001) tissues at a statistically significant level. It was also determined that SOD, GSH-Px activities and GSH levels changed according to tissue characteristics.

Keywords
P. leptodactylus, MDA, SOD, GSH-Px, GSH,

Details
   

Authors

ÖZDEN BARIM ÖZ (1) (Corresponding Author)

FIRAT ÜNİVERSİTESİ
obarimoz@firat.edu.tr | 0000-0003-2914-8167

Bünyamin ÖZDEMİR (2)

221128104@firat.edu.tr | 0009-0007-7815-6821

Supporting Institution

:

Project Number

:

Thanks

:
References
[1] [1]Barım, O., (2005) Keban Baraj Gölü’nde yaşayan Tatlısu istakozu (Astacus leptodactylus Esch. 1823) rasyonuna farklı oranlarda ilave edilen vitamin E’nin etkileri. Fırat Üniv. Fen Bilm. Ens. Doktora Tezi, 73s.

[2] [2]Barim, O., Benzer, F., Erisir, M.ve Dorucu, M., (2009). Oxidant and antioxidant status of tissues of freshwater crayfish (Astacus leptodactylus Esch., 1823) from different stations in the Keban Dame Lake. Fresenius Environmental Bulletin. 18(6): 948-954.

[3] [3]Mazlum, Y. ve Yılmaz, E., (2012). Kerevitlerin biyolojisi ve yetiştiriciliği. Mustafa Kemal Üniv. Yayınları:34. İskenderun-Hatay, 120s.

[4] [4] Wilhelm Filho, D.V., Tribes, T., Gaspari, C., Claudio, F.D., Torres, M.A. ve Magalhaes, A.R.M., (2001) Seasonel changes in antioxidant defenses of the digestive gland of the brown mussel (Perna perna). Aquaculture, 203, 149-158.

[5] [5]Ayala, A., Munoz, M.F. ve Argüelles, S., (2014) Lipid Peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-Hydroxy-0-Nonenal. Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity pp. 31.

[6] [6] Regoli, F. ve Giuliani, M.E., (2014) Oxidative pathways of chemical toxicity and oxidative stress biomarkers in marine organisms. Marine Environmental Research 93, 106-117.

[7] [7]Doğan, S. ve Barim Öz, Ö. (2024). Astaksantin ve su ürünlerinde kullanımı. Serüven Yayınevi, Basım sayısı:1, ISBN:978-625-5955-82-1, 95 -116.

[8] [8] Winston, G.W. ve Guilio, R.T.D., (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquatic Toxicology, 19, 137-161.

[9] [9]Barim, O. ve Karatepe, M., (2010) The effects of pollution on the vitamins A, E, C, beta-carotene contents and oxidative stress of the freshwater crayfish, Astacus leptodactylus. Ecotoxicol Environ Saf 73, 138–142.

[10] [10]Palace, V.P. ve Werner, J., (2006) Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review. Scientia Marina 70S2, 41-57.

[11] [11]Ching, B., Chew, S.F. ve Ip, Y.K., 2015. Ascorbate synthesis in fisheries: A rewiew. International aaunion of Biochemistry and Molecular Biology. 67:2, 69-76.

[12] [12]Dawood, M.A.O. ve Koshio, S., 2018. Vitamin C supplementation to optimize growth, health and stress resistance in aquatic animals. Reviews in Aquaculture. 10:3, 334-350.

[13] [13]Barata, S., Şahin, H. ve Köprücü, S., (2017). Determination of Oxidative Stress Vitamin A E C and B-caroten Levels in the Tissue of Luciobarbus esocinus (Heckel, 1843). Nevşehir Bilim ve Teknoloji Dergisi. 6: 2, 476–482.

[14] [14]Hernandez, L.H. ve Hardy, R.W., 2020. Vitamin A functions and requirements in fish. Aqauculture Research. 51, 3061-3071.

[15] [15]Barim Öz, Ö. ve Doğan, S. (2024). Vitamin A’nin yapisi ve baliklardaki etkileri. Serüven Yayınevi, Basım sayısı:1, ISBN:978-625-5955-44-9, 1 -20.

[16] [16]AOAC (Association of Official Analytical Chemists), (1984) Official methods of analysis (14th Ed), Association of Official Analytical Chemists. Inc. Arlington 1141 p.

[17] [17]Barim, O. ve Erisir, M., (2009) The effect of dietary antioxidants on the arginase activity and nitric oxide level of freshwater crayfish (Astacus leptodactylus, Esch. 1823). Kafkas Univ Vet Fak Derg 15(5), 745-750.

[18] [18] Barim-Oz, O. ve Sahin H., (2016) Oxidative stress and some biochemical parameters during starvation and refeeding in Astacus leptodactylus (Esch., 1823). Cell and Molecular Biology. 62(13),35-43.

[19] [19] Ohkawa, H., Ohishi, N., Yagi, K., (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95, 351-358.

[20] [20] Sun, Y., Oberley, W.L. ve Lı, Y., (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem. 34, 97-500.

[21] [21] Beutler, E., Duron, O. ve Kelly, B.M. (1975) Improved method for the determination of blood glutathione, Journal of Laboratory and Clinical Medicine, 61, 882-888.

[22] [22] Ellman, G.L., (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82 (1), 70-7.

[23] [23] Barım Öz, Ö. ve Barata, S., (2021). Effects of reproduction on oxidative stress and some antioxidant levels in tissues of Capoeta umbla Heckel 1843. Journal of Materials and Electronic Devices, 7–13.

[24] [24] Barım Öz, Ö. ve Barata, S., (2022). Taurin ve su ürünlerinde kullanımı. Eğitim Yayınevi, Basım sayısı:1, ISBN:978-625-6382-12-1, 151 -165.

[25] [25] Paital, B. ve Chainy, G.B.N., (2013) Seasonal variability of antioxidant biomarkes in mud crabs (Scylla serrata). Ecotoxicology and Environmental Safety 87, 33-41.

[26] [26] Verlecar, X.N., Jena K.B. ve Chainy, G.B.N. (2008) Seasonal variation of oxidative biomarkers in gills and digestive gland of green-lipped mussel Perna viridis from Arabian Sea. Estuarine, Coastal and Shelf Science 76, 745-752.

[27] [27] Borkovic, S.S., Pavlovic, S.Z., Kovacevic, T.B., Štajn, A.Š., Petrovic, V.M. ve Saicic, Z.S. (2008). Antioxidant defence enzyme activities in hepatopancreas, gills and muscle of Spiny cheek crayfish (in hepatopancreas, gills and muscle of Spiny cheek crayfish (Orconectes limosus) from the River Danube. Comprative Biochemistry and Physiology Part C 147, 122-128.

[28] [28] Bayir, A., Sirkecioglu, A.N., Haliloglu, H.I., Aksakal, E., Gunes M. ve Aras, N.M., (2011) Influence of season on antioxidant defense systems of Silurus glanis Linnaeus (Siluridae) and Barbus capito capito Güldenstädt (Cyprinidae). Fresenius Environmental Bulletin 20(1), 3-11.

[29] [29] Aiken, D. E. ve Waddy, S.L., (1992). The Growth Process in Crayfish. Reviews in Aquatic Sciences 6(3-4): 335-381.

[30] [30] Yudkovski, Y., Glazer L., Shechter A., Reinhardt R., Chalifa-Caspi V., Sagi A. ve Tom, M., (2010) Multi-transcript expression patterns in the gastrolith disk and the hypodermis of the crayfish Cherax quadricarinatus at premoult. Comperative Biochemistry and Physiology Part D: Genomics and Proteomics. 5(2): 171-177.

[31] [31] Ghanawi, J. ve Saoud, I.P., (2012) Moulting, reproductive biology, and hatchery management of redclaw crayfish Cherax quadricarinatus (von Martens 1868). Aquaculture. 18:358-359.

[32] [32] Da Costa, L.A., Badaw, A. ve El-Sohemy, A., (2012) Nutrigenetics and Modulation of oxidative stres. Ann. Nutr. Metab. 60(3): 27-36.

[33] [33] Pisoschi , A.M. ve Pop, A., (2015). The role of antioxidants in the chemistry of oxidative stres: A review. Europan Journal of Medicinal Chemistry 97: 55-74.

[34] [34]Oost R., Beyer J. ve Vermeulen, N.P.E., (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ. Toxicol. Pharmacol. 13(2):57-149.

[35] [35]Shastak, Y., Gordillo, A. ve Pelletier, W. (2023). The relationship between vitamin A status and oxidative stress in animal production. Journal of Applied Animal Research. 51:1, 546-553.

[36] [36]Barim-Oz, O., Yılmaz, S., Şahin H. ve Benzer, F., (2017). Monthly variability of oxidant and antioxidant biomarkers of freshwater crayfish (Astacus leptodactylus Esch, 1823). Fresenius Environmental Bulletin. 26:4, 3038-3047.

[37] [37] Barim-Oz, O. ve Yilmaz, S., (2009) The determination of effects vitamin E, C, astaxanthin and ß-carotene on oxidative stress in some tissues of freshwater crayfish (Astacus leptodactylus Esch. 1823) in moulting period. E-Journal of New World Sciences Academy 4(3), 39-46.