THE CYTOTOXIC, APOPTOTIC AND NECROTIC EFFECCTS OF SUILLUS COLLINITUS (FR.) KUNTZE

Ferit ARPAZ¹ , Perihan GÜLER² , Mustafa TÜRK³

In the study, cytotoxicity, apoptotic, necrotic effects of Suillus collinitus were investigated. Taken parts from the dry basidiocarps were developed by tissue culture method on malt extract agar. The primary and the secondary mycelium were obtained by incubation at 27°C in the dark for 10 d. Five pellets were taken from the secondary mycelium and were completed their growth in 7 d at 140 rpm at 27°C in nutrient broth. The samples were prepared for cytotoxic, apoptotic, necrotic, studies by extracting at 80°C and 1 h in ethyl alcohol at soxlet device. In cytotoxic studies, sample concentrations for extract MCF-7 and L929 fibroblast cells were applied as 1mg/ml,0.5mg/ml, 0.25mg/ml respectively. The WST-1 solution was added and the absorbance was measured in a microplate reader at a wavelength of 440 nm at the end of the incubation. Apoptosis and necrosis were detected in same cell line as the double staining experiment. Binary staining solution was prepared and MCF-7 and L929 fibroblast fibroblast cells were applied at the same concentrations. As a result of incubation, the measurement was done at fluorescense microscopy at 20X magnification in FITC and DAPI filters. Toksite varies depending on concentrations. At toxicity of 0.5mg/ml showed 86% toxic effect. As concentration decreased, toxicity decreased. In application to fibroblasts, it was determined that it does not show any toxic effect at all applied concentrations.

Keywords
Suillus collinitus , Cancer, Cytotoxicity, Apoptotic Effects, Necrotic Effects,