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Article Details

Article Details

  • Article Code : NWSA-2492-2565
  • Article Type : Araþtýrma Makalesi
  • Publication Number : 3B0019
  • Page Number : 1-6
  • Doi : 10.12739/
  • Abstract Reading : 1284
  • Download : 136
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Issue Details

  • Year : 2012
  • Volume : 7
  • Issue : 1
  • Number of Articles Published : 1
  • Published Date : 1.01.2012

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Dergi Kapaðý
Veterinary Sciences

Serial Number : 3B
ISSN No. : 1308-7339
Release Interval (in a Year) : 4 Issues

ÝZMÝT BÖLGESÝNDE SATIÞA SUNULAN PEYNÝRLERDEKÝ AFLATOKSÝN M1 DÜZEYLERÝNÝN ELISA YÖNTEMÝYLE TESPÝTÝ

Ahmet Hulusi DÝNÇOÐLU 1 , Zafer GÖNÜLALAN2 , Filiz KÖK 3

Bu çalýþma Ýzmit bölgesindeki farklý market ve satýþ noktalarýndan temin edilen beyaz peynir ve kaþar peynirlerinde aflatoksin M1 (AFM1) düzeylerini tespit etmek amacýyla yapýlmýþtýr. 102 adedi beyaz peynir 83 adedi de kaþar peyniri olmak üzere toplam 185 adet peynir örneði incelenmiþtir. Örneklerdeki AFM1 miktarlarýný tespit etmek için ELISA tekniðinden faydalanýlmýþtýr. Aflatoksinlerin temizleme iþlemi r-biofarm Rida®Aflatoksin column (Art.No. R5001/R5002) immunoaffinite kolun ile, AFM1 analizleri ise r-biofarm Rida®Aflatoksin M1 test kitleri (Art.No 1101) kullanýlarak yapýldý. Toplam 185 adet peynir örneðinin 123â€â„¢ünde (%66.5) AFM1 tespit edilmiþtir. Bunlardan 75 adedi (%73.5) beyaz peynirlerde, 48 adedi de (%57.8) kaþar peynirlerinde saptanmýþtýr. Ortalama AFM1 düzeyleri beyaz peynirlerde 189.47 ng/kg, kaþar peynirlerinde ise 229.79 ng/kg miktarýnda tespit edilmiþtir. Beyaz peynirlerde ve kaþar peynirlerinde 16 þar örneðin Türk Gýda Kodeksi sýnýr deðerlerinin (250 ng/k

Keywords
Aflatoksin, Beyaz peynir, Kaþar peyniri, Halk saðlýðý, ELISA,

DETERMÝNATÝON OF AFLATOXÝN M1 LEVELS IN CHEESE SOLD IN ÝZMÝT PROVÝNCE

Ahmet Hulusi DÝNÇOÐLU 1 , Zafer GÖNÜLALAN2 , Filiz KÖK 3

This study was conducted to determine the the AFM1 levels of Turkish white and kashar cheese purchased from different markets and sellers in Ýzmit province. AFM1 was determined by ELISA technique in total of 185 cheese samples including 102 Turkish white and 83 kashar cheese. The aflatoxin clearance was carried out with r-biofarm Rida®Aflatoxin (Art.No. R5001/R5002) immunoaffinity column, AFM1 analyses were performed with r- biofarm Rida®Aflatoxin M1 test kits (Art.No 1101). Of the total of 185 cheese samples, 123 (66.5%) were found AFM1 positive. 75 (73.5%) of positive samples were white cheese samples while 48 (%57.8) of which were kashar cheese. The mean AFM1 levels were 189.47 ng/kg and 229.79 ng/kg for white and kashar cheese respectively. 16 samples belonging to each of white and kashar cheese, exceeded the corresponding maximum permissible limit (250 ng/kg) laid down in Turkish Food Codex.

Keywords
Aflatoxin, White cheese, Kashar cheese, Public health, ELISA,

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Authors

Ahmet Hulusi DÝNÇOÐLU (1) (Corresponding Author)
Harran Üniversitesi Veteriner Fakültesi
adincoglu@harran.edu.tr

Zafer GÖNÜLALAN (2)

Erciyes University
zgonulalan@gmail.com | 0000-0002-3935-6296

Filiz KÖK (3)

filiz_root@yahoo.com

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