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Article Details

Article Details

  • Article Code : FIRAT-AKADEMI-9241-5555
  • Article Type : Araştırma Makalesi
  • Publication Number : 5A0176
  • Page Number : 170-178
  • Doi : 10.12739/NWSA.2022.17.4.5A0176
  • Abstract Reading : 1390
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Issue Details

  • Year : 2022
  • Volume : 17
  • Issue : 4
  • Number of Articles Published : 15
  • Published Date : 1.10.2022

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Dergi Kapağı
Ecological Life Sciences

Serial Number : 5A
ISSN No. : 1308-7258
Release Interval (in a Year) : 4 Issues

Sibel DOĞAN 1 , Şükrü ÖNALAN2

Keywords

EFFECTS OF PRESERVATION CONDITIONS ON DNA QUALITY TO BE USED IN PARASITIC STUDIES IN FISHERIES

Sibel DOĞAN 1 , Şükrü ÖNALAN2

Among the parasitic disease agents, Diplostomums are among the most common and highly pathogenic agents. In this study, DNA isolation and subsequent purity and concentration differences were investigated from parasite samples taken under different stock conditions. In the concentration differences, it was observed that the concentrations of the parasite samples kept at -20°C without buffer and the samples kept in 0.9% saline at -20°C were insignificantly higher (p<0.05). According to the results of the study, if the starting material is DNA, it has been observed that there is no problem in terms of DNA concentration if the tissue samples are kept at -20°C in a short time (3 days) without the need for a buffer solution.

Keywords
Fish, Fish Parasites, Diplostomum sp., DNA Isolation, Storage Conditions,

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Authors

Sibel DOĞAN (1) (Corresponding Author)
Fırat University
sbarata@firat.edu.tr | 0000-0003-4569-5435

Şükrü ÖNALAN (2)

sukruonalan@yandex.com | 0000-0003-0058-5232

Supporting Institution

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Project Number

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Thanks

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